Pengaruh Ultrafine Bubbles dan Dark Septate Endophytes terhadap Pertumbuhan Bibit Malapari (Pongamia pinnata)
Effect of Ultrafine bubbles and Dark Septate Endophytes on the Seedling Growth of Malapari (Pongamia pinnata)
DOI:
https://doi.org/10.59465/jpht.v21i2.383Kata Kunci:
Malapari, dark septate endophytes, daya berkecambah, ultrafine bubblesAbstrak
Abstract
Malapari has great potential to produce biofuel, and is known as an energy source plant that many stakeholders are developing. The Malapari planting program needs information related to germination and how to produce seedlings that are ready for planting, have vigor, and can adapt to the planting area. This research aims to determine the effect of treatment with ultrafine bubbles (UFB) water and dark septate endophytes (DSE) fungi, which are invigoration technologies to increase the viability and vigor of malapari seeds and seedlings. The research design used a completely randomized factorial design with two factors, namely treatment of soaking seeds with UFB water with doses consisting of 0, 8 ppm, and 20 ppm, and DSE treatment using without DSE, DSE Dendrothyrium sp., DSE Cladophialophora nyingchiensis, and DSE Exophiala pisciphila. The results showed that malapari seed moisture content of 9.36% gave a high germination percentage, then the soaking of UFB water, which was not an effective method to be used. Giving DSE Exophiala pisciphila fungi to the seeds could maintain their germination percentage and improve the germination rate. In addition, it accelerates the mean of germination days and mean daily germination, furthermore, the seedling were studied more, resulting the higher seedling quality.
Keywords: Malapari, dark septate endophytes, seed germination, ultrafine bubbles
Abstrak
Malapari memiliki potensi yang besar untuk menghasilkan minyak nabati, dan dikenal sebagai tanaman sumber energi yang sedang dikembangkan oleh banyak pihak. Program penanaman jenismalapari perlu didukung informasi perkecambahan dan pembibitan untuk menghasilkan bibit yang siap tanam, vigor dan mampu beradaptasi di lahan penanaman. Penelitian bertujuan untuk menganalisis efektivitas pemberian air ultrafine bubbles (UFB) dan cendawan dark septate endophytes (DSE) sebagai salah satu teknologi invigorasi g meningkatkan viabilitas dan vigor benih, serta bibit malapari. Rancangan penelitian menggunakan faktorial dalam rancangan acak lengkap dengan dua faktor, yaitu faktor perendaman benih dengan UFB water terdiri 3 taraf (dosis 0 sebagai kontrol, 8 ppm, dan 20 ppm) dan faktor DSE 0,1% terdiri 4 taraf (kontrol, DSE Dendrothyrium sp., DSE Cladophialophora nyingchiensis, dan DSE Exophiala pisciphila). Hasil penelitian menunjukkan bahwa benih malapari
dengan kadar air 9,36% masih mempunyai daya berkecambah yang tinggi sehingga tidak efektif diberi perlakuan perendaman dengan UFB. Benih malapari yang diberi cendawan DSE E. pisciphila bisa mempertahankan daya berkecambah benih dan dapat meningkatkan kecepatan perkecambahannya. Cendawan DSE E. pisciphila juga mempercepat rata-rata hari berkecambah, meningkatkan perkecambahan harian, bibit lebih kokoh, dan indeks mutu bibit lebih tinggi
Kata kunci: Malapari, dark septate endophytes, daya berkecambah, ultrafine bubbles
Referensi
Ahmed, A. K. A., Shi, X., Hua, L., Manzueta, L., Qing, W., Marhaba, T., & Zhang, W. (2018). Influences of Air, Oxygen, Nitrogen, and Carbon Dioxide Nanobubbles on Seed Germination and Plant Growth. Journal of Agricultural and Food Chemistry, 66(20), 5117–5124. https://doi.org/10.1021/acs.jafc.8b00333
Alimah, D. (2011). Budidaya dan potensi malapari (Pongamia pinnata l. pierre) sebagai tanaman penghasil bahan bakar nabati. Galam, 5(1), 35–49.
Aminah, A., & Syamsuwida, D. (2013). Penentuan karakteristik fisiologis benih kranji (Pongamia pinnata) berdasarkan nilai kadar air. Jurnal Penelitian Hutan Tanaman, 10(1–6).
Arpiwi, N. L., Yan, G., Barbour, E. L., Plummer, J. A., & Watkin, E. (2013). Phenotypic and genotypic characterisation of root nodule bacteria nodulating Millettia pinnata (L.) Panigrahi, a biodiesel tree. Plant and Soil, 367(1–2), 363–377. https://doi.org/10.1007/s11104-012-1472-4
Asih, P. R. (2021). Invigorasi Mutu Fisiologis Benih Terung Ungu (Solanum melongena L.) Kadaluarsa dengan Beberapa Teknik Osmoconditioning. Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science), 18(2), 162–170. https://doi.org/10.32528/agritrop.v18i2.3905
Dalimunthe, C. I. S. B. P. W., Munif, A., & Surono. (2019). Seleksi dan uji potensi cendawan dark septate endophyte sebagai agensia hayati penyakit jamur akar putih (Rigidoporus microporus) pada tanaman karet. Penelitian Karet, 37(1), 11–20.
Deswal, R. P. S., Kaushik, N., Panwar, N., Rawat, L., & Bangarwa, K. S. (2015). Seed viability and oil content in seeds of Pongamia pinnata of five sources stored at different moisture and temperature regimes. Research on Crops, 16(3), 568–578.
Diaz-Vivancos, P., Barba-Espín, G., & Hernández, J. A. (2013). Elucidating hormonal/ROS networks during seed germination: Insights and perspectives. Plant Cell Reports, 32(10), 1491–1502. https://doi.org/10.1007/s00299-013-1473-7.
Dickson, A., Leaf, A. L., & Hosner, J. F. (1960). Quality Appraisal of White Spruce and White Pine Seedling Stock in Nurseries. The Forestry Chronicle, 36(1), 10–13. https://doi.org/10.5558/tfc36010-1
Fatchullah, D. (2017). Pengaruh kerapatan tanaman terhadap pertumbuhan dan hasil benih kentang (Solanum tuberosum L.) generasi satu (G1) varietas granola. Planta Tropika: Journal of Agro Science, 5(1), 15–22. https://doi.org/10.18196/pt.2017.067.15-22
Fatikhasari, Z., Lailaty, I. Q., Sartika, D., & Ubaidi, M. A. (2022). Viabilitas dan vigor benih kacang tanah (Arachis hypogaea L.), kacang hijau (Vigna radiata (L.) R. Wilczek), dan jagung (Zea mays L.) pada temperatur dan tekanan osmotik berbeda. Jurnal Ilmu Pertanian Indonesia, 27(1), 7–17. https://doi.org/10.18343/jipi.27.1.7
Febritasari, F., Arpiwi, N. L., & Wahyuni, I. G. A. S. (2016). Karakteristik dan analisis hubungan kekerabatan malapari(Pongamia pinnata (l)pierre ) sebagai tanaman penghasil minyak disua aksesi. Jurnal Metamorfosa, 3(2), 74–81.
Haase, D. L. (2008). Understanding forest seedling quality: measurements and interpretation. Tree Planters’ Notes, 52(2), 24–30. http://forestseedlingnetwork.com/media/15912/Understanding_Forest_Seedling_Quality_-_Measurements_and_Interpretation.pdf
Handayani, D. (2017). Karakteristik Cendawan Dark Septate Endophyte (Dse) Pada Akar Tanaman Jagung Dan Padi. EKSAKTA: Berkala Ilmiah Bidang MIPA, 18(01), 61–68. https://doi.org/10.24036/eksakta/vol18-iss01/20
Hasanah, U., Riwayati, & Idramsa. (2015). Uji Antijamur Patogen Ekstrak Metabolit Sekunder Jamur Endofit Tumbuhan Raru (Cotylelobium Melanoxylon). Jurnal Biosains, 1(2).
He, C., Wang, W., & Hou, J. (2019). Characterization of dark septate endophytic fungi and improve the performance of liquorice under organic residue treatment. Frontiers in Microbiology, 10(JUN), 1–14. https://doi.org/10.3389/fmicb.2019.01364
Hong, T. D., & Ellis, R. H. (1996). A protocol to determine seed storage behaviour. In International Plant Genetic Resources Institute. https://doi.org/10.3390/plants9050581
Indriani, F. . H. S. R. S. (1999). Pengaruh Invigorisasi Terhadap Benih dan Pertumbuhan Tanaman Kenaf (Hibiscus cannabinus L.). In Jurnal Litri: Vol. IV (Issue 01, pp. 1–5).
Kumar, S., Radhamani, J., Singh, A. K., & Varaprasad, K. S. (2007). Germination and seed storage behaviour in Pongamia pinnata L. [12]. Current Science, 93(7), 910–911.
Li, M., He, C., Wei, M., Long, J., Wang, J., Yang, X., Wang, K., & He, X. (2024). Temporal and spatial dynamics and functional metabolism of dark septate endophytes of Gymnocarpos przewalskii Maxim. in Northwest Desert, China. Applied Soil Ecology, 194(November 2023), 105194. https://doi.org/10.1016/j.apsoil.2023.105194
Maia, J., Qadir, A., Widajati, E., & Purwanto, Y. A. (2021). Ultrafine Bubbles Technology for Breaking Dormancy of Sandalwood Seeds (Santalum album L. Jurnal Perbenihan Tanaman Hutan, 9(1), 27–41. https://doi.org/10.20886/bptpth.2021.9.1.27-41.
Masilewi, J., Nurdin, A. S., Marasabessy, M. H., Irmayanti, L., Ashari, R., & Kehutanan, P. S. (2022). Pertumbuhan jabon merah (Anthocepalus macrophyllus ) pada komposisi media tanam yang berbeda. Ulin - J Hut Trop, 6(1), 98–104.
Nurhasybi, Sudrajat, D. J., & Suita, E. (2019). Tree Seedling Nursery Stock Criteria: for Forest Plantation Establisment and Land Rehabilitation.
Pithaloka, S. A., Sunyoto, Kamal, M., & Hidayat, K. F. (2015). Pengaruh kerapatan tanaman terhadap pertumbuhan dan hasil beberapa varietas sorgum (Sorghum bicolor (L.) Moench). Jurnal Agrotek Tropika, 3(1), 56–63. https://doi.org/10.23960/jat.v3i3.1957
Poovarasan, T., Jerlin, R., Kennedy, J.S., Senthil, N., Sasthri, G., & Anand, T. 2022. Seed biopriming by endophytes for enhanced field performance of hybrid maize (Zea mays L.) COH(M) 8. Agricultural Science Digest, 1-6.
Rajagukguk, J. A., Setiadi, Y., Hilwan, I., & Mardatin, N. F. (2019). Respon Pertumbuhan Bibit Gmelina Arborea Roxb. Terhadap Perlakuan Media Tanam dan Biostimulan di Persemaian Permanen IPB. Journal of Tropical Silviculture, 10(1), 15–20. https://doi.org/10.29244/j-siltrop.10.1.15-20
Rahman, R.S. 2022. Kombinasi perlakuan ultrafine bubbles dan dark septate endophytes untuk perbaikan viabilitas dan vigor benih krasikarpa (Acacia crassicarpa A. Cunn. Ex Benth.). Skripsi. Institut Pertanian Bogor.
Siregar, I., Ramdhani, R., Rustam, E., & Sudrajat, D. (2021). The Effect of Invigoration Using Polyethylene Glycol And Ultra Fine Bubble on Improving of Sengon Seeds (Falcataria Moluccana Miq.) Quality After Two Years Storage. Jurnal Wasian, 8(2), 133–143. https://doi.org/10.20886/jwas.v8i2.5997
Siregar, I. Z., Muharam, K. F., Purwanto, Y. A., & Sudrajat, D. J. (2020). Seed germination characteristics in different storage time of gmelina arborea treated with ultrafine bubbles priming. Biodiversitas, 21(10), 4558–4564. https://doi.org/10.13057/biodiv/d211013
Sudrajat, D. J., Nurhasybi, N., & Syamsuwida, D. (2011). TEKNOLOGI UNTUK MEMPERBAIKI PERKECAMBAHAN BENIH KEPUH ( Linn.). Jurnal Penelitian Hutan Tanaman, 8(5), 301–314. https://doi.org/10.20886/jpht.2011.8.5.301-314
Sudrajat, D. J., Putri, A., Purwanto, Y. A., & Siregar, I. Z. (2022). Effectiveness of ultrafine bubbles and gamma irradiation treatments to improve seed viability and vigor of Albizia chinensis (Osbeck) Merr. Journal of Forestry Research, 33(5), 1607–1613. https://doi.org/10.1007/s11676-021-01442-5
Sudrajat, D. J., Siregar, I. Z., & Purwanto, Y. A. (2022). Teknologi ULTRAFINE BUBBLES ( UFB ). IPB Press.
Sudrajat, D. J., Yuniarti, N., Nurhasybi, Syamsuwida, D., Danu, Pramono, A. A., & Putri, K. P. (2017). Karakteristik dan Prinsip Penanganan Benih Tanaman Hutan Berwatak Intermediet dan Rekalsitran (Issue December).
Sudrajat, D. J., Yuskianti, V., Siregar, I. Z., & Rustam, E. (2024). Teknologi Penanganan Benih dan Bibit Spesies Potensial untuk Restorasi Hutan dan Lanskap di Indonesia. Dee Publish.
Suita, & Syamsuwida. (2016). Pengaruh pengeringan terhadap viabilitas benih malapari (. Jurnal Perbenihan Tanaman Hutan, 4(1), 9–16.
Suita, E & Syamsuwida (2015). Peningkatan daya dan kecepatan berkecambah benih malapari (Pongamia pinnata). Jurnal Perbenihan Tanaman Hutan, 3(1), 43–50.
Surono, & Narisawa, K. (2017). The dark septate endophytic fungus Phialocephala fortinii is a potential decomposer of soil organic compounds and a promoter of Asparagus officinalis growth. Fungal Ecology, 28(October), 1–10.
https://doi.org/10.1016/j.funeco.2017.04.001
Surono, & Narisawa, K. (2018). The inhibitory role of dark septate endophytic fungus Phialocephala fortinii against Fusarium disease on the Asparagus officinalis growth in organic source conditions. Biological Control, 121, 159–167. https://doi.org/10.1016/j.biocontrol.2018.02.017
Sutopo, L. (2002). Teknologi Benih. PT Raja Grafindo Persada.
Timotiwu, P. B., Nurmauli, N., & Khairunnisa, F. H. (2022). Studi Mengenai Viabilitas Dan Vigor Benih Kedelai Baru Dan Lama. Jurnal Agrotek Tropika, 10(4), 541. https://doi.org/10.23960/jat.v10i4.6450
Widajati, E. (2013). Metode Pengujian Mutu Benih. In Dasar Ilmu dan Teknologi Benih (Pertama, pp. 109–148). IPB Press.
Widodo, T. W., Damanhuri, D., Muhklisin, I., & Titale, I. A. (2022). Production of 3 Varieties of Rice (Oryza sativa L.) on Soilless Farming System Based on Intermittent Irrigation as Urban Farming Method. Jurnal Ilmiah Inovasi, 22(2), 184–193. https://doi.org/10.25047/jii.v22i2.3352
Yuniarti, N., Nurhasybi, & Darwo. (2016). Karakteristik benih kayu bawang (Azadirachta excelsa (Jack) Jacobs) berdasarkan tingkat pengeringan dan ruang penyimpanan. Jurnal Penelitian Hutan Tanaman, 13(2), 105–112.
Zakia, A., Ulum, M. B., Iriany, A., & Zainudin, A. (2021). Modifikasi Teknik Invigorasi untuk Meningkatkan Viabilitas dan Vigor Benih Jagung Manis (Zea mays Sacharata L.). Agriprima : Journal of Applied Agricultural Sciences, 5(1), 50–60. https://doi.org/10.25047/agriprima.v5i1.383