STERILIZATION AND INDUCTION CALLUS OF KLUWIH TEAK (Tectona grandis f. abludens) USING IN VITRO CULTURE
DOI:
https://doi.org/10.59465/ijfr.2026.13.2.283-300Keywords:
Tectona grandis f. abludens, kluwih teak, in vitro, sterilization, embryogenic callus inductionAbstract
Tectona grandis f. abludens or kluwih teak is a variety of teak endemic to Java, especially found in Desa Selang Gunung Kidul and Desa Loh Putih, Kecamatan Dlingo Bantul -Yogyakarta, where currently only six trees are found. Due to very low population size of this teak variety, efforts to conserve them are indispensable. The action conservation was achieved through in vitro propagation methods (embryogenesis). However, the main problems with in vitro techniques are known especially with explant sterilization and callus induction. This study aimed to analyze the response of mercury chloride (HgCl2) immersion time to explant sterilization and analyze the response of a combination of 2,4-D Plant Growth Regulators (PGR) and kinetin to embryogenic callus formation. The data analysis method used a Completely Randomised Design (CRD) for explants sterilization consisting of 300 mg/L HgCl2 treatment in 0; 15; 20; 25 and 30 minutes and Complete Randomised Factorial Design (CFRD) consisting of 2,4-D: 0; 1.5; 3 mg/L and kinetin: 0; 0.5; 1; 1.5 mg/L for callus induction. The results of the explant sterilization study showed that immersion treatment of 300 mg /L HgCl2 for 15 minutes resulted in the highest sterile explants of 90% at 15 day after planting (DAP). The results of callus induction research showed that 0 ppm 2,4-D treatment combined with 1.5 mg/L kinetin resulted in the highest callus percentage of 73.33% and the highest callus formation score was 1.53 at 12 WAP (week after planting).
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